124 research outputs found

    La mancha bacteriana de los frutales de hueso y del almendro (Xanthomonas arboricola pv. pruni): una enfermedad emergente

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    CYTED. Programa Iberoamericano de Ciencia y Tecnología para el Desarroll

    Aspectos fitosanitarios del cultivo del azafrán del Jiloca: enfermedades de etiología bacteriana

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    Proyecto INIA PET 2007-14-C05-0

    Enfermedades bacterianas de la patata: situación en Aragón

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    Diagnosis and detection of the main bacterial pathogens of stone fruit and almond

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    Diagnosis and detection are key aspects related to plant health status. A critical review of the available diagnostic methods utilised for Agrobacterium rhizogenes and A. tumefaciens, Pseudomonas amygdali, P. yringae pv. mors-prunorum, P. syringae pv. persicae, P. syringae pv. syringae and Xanthomonas arboricola pv. pruni, the main pathogens of the stone fruit trees, is presented. As there is a general lack of updated standardized protocols for the detection of most of these bacteria, the most appropriate media for their isolation are reported along with serological reagents, PCR and real-time PCR protocols with comments on their accuracy for the analysis of these pathogens in plant samples. There are many selective media for isolation, especially for Agrobacterium spp., but fewer for Pseudomonas spp. and X. arboricola pv. pruni. Serological techniques are not very useful for these pathogens due to the current lack of specific antibodies commercially available. As to molecular methods, it is surprising to find so many PCR protocols for Agrobacterium species, very few and unspecific for the Pseudomonas species pathogenic to stone fruit trees, and several recent PCR protocols for X. arboricola pv. pruni. The new advances in genomics and proteomics will provide information for selecting new targets to develop specific and sensitive techniques for the diagnosis and detection of these bacterial pathogens in plant material.The authors thank the contributions of the participants in the EU-COST Action 873 and the project RF2009-00002-C04-01 “Prospección, ecolección, conservación y caracterización de nuevo germoplasma de melocotonero”

    Secuencia del genoma de una cepa española de Xanthomonas arboricola pv. pruni aislada de almendro

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    PublishedTrabajo financiado por el Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, proyecto RTA2011-00140-d0T-0

    Draft Genome Sequence of Two Strains of Xanthomonas arboricola Isolated from Prunus persica Which Are Dissimilar to Strains That Cause Bacterial Spot Disease on Prunus spp.

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    The draft genome sequences of two strains of Xanthomonas arboricola, isolated from asymptomatic peach trees in Spain, are reported here. These strains are avirulent and do not belong to the same phylogroup as X. arboricola pv. pruni, a causal agent of bacterial spot disease of stone fruits and almonds.This work was supported financially by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) project RTA2014- 00018-CO2-01.Publishe
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